human cardiac microvasculature endothelial cells Search Results


90
Cleaver Scientific huvec culture medium
Huvec Culture Medium, supplied by Cleaver Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cayman Chemical anti-human endothelial nitric oxide synthase (enos) rabbit polyclonal antibody
Anti Human Endothelial Nitric Oxide Synthase (Enos) Rabbit Polyclonal Antibody, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti-human endothelial nitric oxide synthase (enos) rabbit polyclonal antibody - by Bioz Stars, 2026-08
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Becton Dickinson human aortic endothelial lysate (r2β
Human Aortic Endothelial Lysate (R2β, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human aortic endothelial lysate (r2β - by Bioz Stars, 2026-08
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Lonza 26 lung-derived normal human microvascular endothelial cells (hmvecs) were purchased
26 Lung Derived Normal Human Microvascular Endothelial Cells (Hmvecs) Were Purchased, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/pmc02993283-54-3-11?v=Lonza
Average 90 stars, based on 1 article reviews
26 lung-derived normal human microvascular endothelial cells (hmvecs) were purchased - by Bioz Stars, 2026-08
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SwitchGear Genomics vegf-b reporter construct containing the 5′ flanking region (−851 to +156) of human vegf-b
Vegf B Reporter Construct Containing The 5′ Flanking Region (−851 To +156) Of Human Vegf B, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
vegf-b reporter construct containing the 5′ flanking region (−851 to +156) of human vegf-b - by Bioz Stars, 2026-08
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Thermo Fisher gene exp pecam1 hs01065279 m1
Gene Exp Pecam1 Hs01065279 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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Biomol GmbH human recombinant s1p
Human Recombinant S1p, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant s1p - by Bioz Stars, 2026-08
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Beijing Solarbio Science lipopolysaccharide (huvecs)
Mice, cell lines, and reagents.
Lipopolysaccharide (Huvecs), supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/pmc11973065-9-0-3?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
lipopolysaccharide (huvecs) - by Bioz Stars, 2026-08
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GenScript corporation human epcr cdna
(A) The canonical (left) and non-canonical <t>EPCR</t> structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.
Human Epcr Cdna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/bio_rxiv__2021__12__16__472967-54-0-3?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
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Ribozyme Pharmaceuticals systemically delivered hhrzs angiozyme
(A) The canonical (left) and non-canonical <t>EPCR</t> structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.
Systemically Delivered Hhrzs Angiozyme, supplied by Ribozyme Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/bio_rxiv__2022__04__26__489422-189-6-0?v=Ribozyme+Pharmaceuticals
Average 90 stars, based on 1 article reviews
systemically delivered hhrzs angiozyme - by Bioz Stars, 2026-08
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85
Thermo Fisher gene exp vegfa rh02621759 m1
(A) The canonical (left) and non-canonical <t>EPCR</t> structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.
Gene Exp Vegfa Rh02621759 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/pmc02850229-114-89-6?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp vegfa rh02621759 m1 - by Bioz Stars, 2026-08
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96
New England Biolabs virus strains neb turbo competent e coli
(A) The canonical (left) and non-canonical <t>EPCR</t> structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.
Virus Strains Neb Turbo Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cardiac+microvasculature+endothelial+cells/pm36384122-254-167-176?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
virus strains neb turbo competent e coli - by Bioz Stars, 2026-08
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Image Search Results


Mice, cell lines, and reagents.

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: Mice, cell lines, and reagents.

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: Over Expression, Knockdown, Cell Culture, Staining, cDNA Synthesis, SYBR Green Assay, Real-time Polymerase Chain Reaction, Membrane

(A) The expression levels of CMTM3 transcripts in human umbilical vein endothelial cells (HUVECs) treated with 100 ng/ml LPS for 2h, 6h, and 24h ( n ≧ 3). (B) The expression levels of CMTM3 transcripts in HUVECs subjected to stimulation with 4h-hypoxia/2h-reoxygenation and 5h-hypoxia/2h-reoxygenation. Unstimulated HUVECs were used as the control ( n ≧ 3).

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: (A) The expression levels of CMTM3 transcripts in human umbilical vein endothelial cells (HUVECs) treated with 100 ng/ml LPS for 2h, 6h, and 24h ( n ≧ 3). (B) The expression levels of CMTM3 transcripts in HUVECs subjected to stimulation with 4h-hypoxia/2h-reoxygenation and 5h-hypoxia/2h-reoxygenation. Unstimulated HUVECs were used as the control ( n ≧ 3).

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: Expressing, Control

CMTM3 overexpression enhances permeability of the human umbilical vein endothelial cells (HUVECs) under in-vitro ADRS inflammatory conditions ( n ≧ 3). (A) CMTM3 expression levels in control HUVECs as well as adsham and adCMTM3-transfected HUVECs. (B) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the adsham- and adCMTM3-transfected HUVECs that were untreated or treated with 100 ng/ml LPS for 6h. (C) FITC-Dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the adsham- and adCMTM3-transfected HUVECs that were untreated or treated with 4h-hypoxia/2h-reoxygenation.

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: CMTM3 overexpression enhances permeability of the human umbilical vein endothelial cells (HUVECs) under in-vitro ADRS inflammatory conditions ( n ≧ 3). (A) CMTM3 expression levels in control HUVECs as well as adsham and adCMTM3-transfected HUVECs. (B) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the adsham- and adCMTM3-transfected HUVECs that were untreated or treated with 100 ng/ml LPS for 6h. (C) FITC-Dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the adsham- and adCMTM3-transfected HUVECs that were untreated or treated with 4h-hypoxia/2h-reoxygenation.

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: Over Expression, Permeability, In Vitro, Expressing, Control, Transfection, Fluorescence

CMTM3 knockdown reduces human umbilical vein endothelial cell (HUVEC) permeability under in-vitro inflammatory conditions. (A) CMTM3 expression levels in the control, shsham, and shCMTM3 HUVECs. (B) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the shsham and shCMTM3-transfected-HUVECs that were untreated or treated with 100 ng/ml LPS for 6h. (C) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) of shsham and shCMTM3-transfected HUVECs that were untreated or treated with 4h-hypoxia/2h-reoxygenation.

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: CMTM3 knockdown reduces human umbilical vein endothelial cell (HUVEC) permeability under in-vitro inflammatory conditions. (A) CMTM3 expression levels in the control, shsham, and shCMTM3 HUVECs. (B) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) in the shsham and shCMTM3-transfected-HUVECs that were untreated or treated with 100 ng/ml LPS for 6h. (C) FITC-dextran assay results show the cellular permeability based on relative fluorescence leakage (RFI) of shsham and shCMTM3-transfected HUVECs that were untreated or treated with 4h-hypoxia/2h-reoxygenation.

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: Knockdown, Permeability, In Vitro, Expressing, Control, Fluorescence, Transfection

The expression levels of IL-6 and TNF-α in the adCMTM3- and shCMTM3-transfected HUVECs after (A, C) LPS stimulation for 6 h and (B, D) 4h/2h hypoxia/reoxygenation treatment.

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: The expression levels of IL-6 and TNF-α in the adCMTM3- and shCMTM3-transfected HUVECs after (A, C) LPS stimulation for 6 h and (B, D) 4h/2h hypoxia/reoxygenation treatment.

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: Expressing, Transfection

RNA sequencing analysis of lipopolysaccharide (LPS)–treated shCMTM3-HUVECs. (A) Top 20 KEGG pathways representing DEGs between shCMTM3-HUVECs and shsham- human umbilical vein endothelial cells (HUVECs). (B) Top 20 KEGG pathways between LPS-treated shCMTM3-HUVECs and LPS-treated shsham-HUVECs. (C) PPI network analysis of DEGs between shCMTM3-HUVECs and shsham-HUVECs. (D) PPI network analysis of DEGs between LPS-treated shCMTM3-HUVECs and LPS-treated shsham -HUVECs.

Journal: Frontiers in Immunology

Article Title: CMTM3 regulates vascular endothelial cell dysfunction by influencing pulmonary vascular endothelial permeability and inflammation in ARDS

doi: 10.3389/fimmu.2025.1544610

Figure Lengend Snippet: RNA sequencing analysis of lipopolysaccharide (LPS)–treated shCMTM3-HUVECs. (A) Top 20 KEGG pathways representing DEGs between shCMTM3-HUVECs and shsham- human umbilical vein endothelial cells (HUVECs). (B) Top 20 KEGG pathways between LPS-treated shCMTM3-HUVECs and LPS-treated shsham-HUVECs. (C) PPI network analysis of DEGs between shCMTM3-HUVECs and shsham-HUVECs. (D) PPI network analysis of DEGs between LPS-treated shCMTM3-HUVECs and LPS-treated shsham -HUVECs.

Article Snippet: Lipopolysaccharide (HUVECs) , Solarbio , IL2020.

Techniques: RNA Sequencing

(A) The canonical (left) and non-canonical EPCR structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.

Journal: bioRxiv

Article Title: Structure of EPCR in a non-canonical conformation

doi: 10.1101/2021.12.16.472967

Figure Lengend Snippet: (A) The canonical (left) and non-canonical EPCR structures are shown in grey and paleyellow colors, respectively. The residues in the α2 helix with severe folding transitions are highlighted. 2Fo-Fc electron density maps are shown as blue meshes. In order to confirm the novel conformation, maps were generated using either the canonical or non-canonical coordinates of residues Tyr154 through Thr157. The bound phospholipids are shown in the central cavities in stick format (B) Superposition of the canonical and non-canonical structures. Tyr154, Asn155 and Thr157 are highlighted for comparison purposes. (C) Directional switch in the α2 helix of EPCR.

Article Snippet: Human EPCR cDNA (Genscript) was PCR amplified and cloned in frame with a GP64 signal peptide in a pAcGP67A transfer vector, using BamHI and NotI restriction enzymes and OptizymeTM T4 DNA ligase (Thermo Fisher Scientific).

Techniques: Generated, Comparison

(A) Structure of the protein C Gla domain in complex with the canonical conformation of EPCR (PDB 1LQV). The contacts with the Gla domain established by EPCR Tyr154 are highlighted with grey dashed lines. Tyr154 residues in both the canonical and non-canonical EPCR structures are highlighted in sticks for comparison purposes and to better visualize the impact of the folding transition in protein C binding. (B) Upper panel, measurement of kinetic constant rates and affinity interaction between wild type EPCR and APC (upper panel). Red color traces denote buffer signal-substracted raw binding data and black traces indicate fitting to a 1:1 binding kinetic model. Lower panel, comparison of binding signal of 125 nM APC to EPCR or EPCR Y154A . (C) Upper panel, intermolecular contacts between the non-canonical EPCR Tyr154 and N-acetylglucosamine (NAG) in a crystallographic symmetry mate EPCR molecule. Lower panel, analogous view with the canonical EPCR structure (PDB 1L8J). The 1L8J symmetry mate (palecyan color) is shown superposed with the symmetry mate of the non-canonical EPCR structure.

Journal: bioRxiv

Article Title: Structure of EPCR in a non-canonical conformation

doi: 10.1101/2021.12.16.472967

Figure Lengend Snippet: (A) Structure of the protein C Gla domain in complex with the canonical conformation of EPCR (PDB 1LQV). The contacts with the Gla domain established by EPCR Tyr154 are highlighted with grey dashed lines. Tyr154 residues in both the canonical and non-canonical EPCR structures are highlighted in sticks for comparison purposes and to better visualize the impact of the folding transition in protein C binding. (B) Upper panel, measurement of kinetic constant rates and affinity interaction between wild type EPCR and APC (upper panel). Red color traces denote buffer signal-substracted raw binding data and black traces indicate fitting to a 1:1 binding kinetic model. Lower panel, comparison of binding signal of 125 nM APC to EPCR or EPCR Y154A . (C) Upper panel, intermolecular contacts between the non-canonical EPCR Tyr154 and N-acetylglucosamine (NAG) in a crystallographic symmetry mate EPCR molecule. Lower panel, analogous view with the canonical EPCR structure (PDB 1L8J). The 1L8J symmetry mate (palecyan color) is shown superposed with the symmetry mate of the non-canonical EPCR structure.

Article Snippet: Human EPCR cDNA (Genscript) was PCR amplified and cloned in frame with a GP64 signal peptide in a pAcGP67A transfer vector, using BamHI and NotI restriction enzymes and OptizymeTM T4 DNA ligase (Thermo Fisher Scientific).

Techniques: Comparison, Binding Assay